High Definition For Soluble Lyophilized Silk Fibroin for United States Manufacturer

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High Definition For Soluble Lyophilized Silk Fibroin for United States Manufacturer Detail:

冻干粉溶解过程500

Brand Name: Fancci

Alternative Name: Freeze-dried silk fibroin

Introduction:

Large molecular weight silk fibroin in aqueous solution can be processed into different solid material formats. Unfortunately, the purified silk solution can’t be stored or transported for a long time as silk undergoes structural changes and aggregates due to thermodynamic instability. Our proprietary lyophilized silk product completely solve these problems and the lyophilized silk can be dissolved in water or buffer at high concentration, facilitating downstream material processing.

Lyophilized silk in a glass vial presents to be a white porous sponge. Since the vial is completely sealed, lyophilized silk can remain stable for a long period of time (> 18 months) at room temperature in terms of appearance, solubility, molecular weight distribution and structure.  The regenerated silk solution remains the same structural transition capability from random coil andα-helix structure to β-sheet structure as original silk solution, which is critical in preparation of solid material formats.

Table 1 Parameter, testing and method of lyophilized silk fibroin

Parameter, Testing and Method Lyophilized Silk Fibroin
Category Materials/Agents
Form Lyophilized agents
Source Degummed Bombyx mori silkworm
Package 20 ML Penicillin Bottles 
Method of storage  Stored in a dry, ventilated place at room temperature
Solution concentration after rehydration ≤ 40%
Bioburden Sterilized
Molecular Weight 200,000 Da
Expiration Date 18 months

Operation method:

Lyophilized silk fibroin can be stored at room temperature for 18 months. Before use, water or buffer or cell/drug solution can be added into the sterile glass bottle using a needle syringe (keep sealed) or a pipette (open bottle). The lyophilized silk cake in the bottle can be quickly rehydrated forming a light yellow solution and the silk concentrations obtained can reach up to 30-40%.

Application:

Same as the solution after purification, the soluble lyophilized silk fibroin can also be used as a basic raw material for fabrication of many biomedical products. Furthermore, soluble lyophilized silk fibroin has advantages of longer and ambient storage, easier transportation and higher concentration of regenerated solution compare to regular silk fibroin solution.


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