Qhibilihang ka har'a Lyophilized Silk protheine ena
Short Tlhaloso:
sehlahisoa sena ka boloka tsitsitseng likhoeli tse fetang 18 ka mocheso kamore. The nkeloa sebaka silika tharollo ntse e tšoanang mehaho phetoho bokgoni ho tloha tšohanyetso kela andα-Helix sebopeho ho β-lakane sebopeho ka pele silika tharollo.
B Lebitso ranta: Fancci
Lebitso mefuta e meng: hatsele-omisitsoeng silika protheine
Selelekela :
Kholo ea limolek'hule boima silika protheine ka tharollo ea metsi ka sebetswa ka rulaganya fapaneng tiileng tse bonahalang. Ka bomalimabe, ho hloekisoa silika tharollo ke ke ho bolokoa kapa isoa bakeng sa nako e telele e le silika undergoes liphetoho mehaho le aggregates ka lebaka la ho se tsitse ha thermodynamic. lyophilized rona dikhampani le silika sehlahisoa ho feletseng ho rarolla mathata a bana le silika lyophilized ka qhalwa ho ya ka metsi kapa Buffer ka mahloriso phahameng, bebofatswe tlaase ho linoka tse bonahalang ho e lokisa.
Lyophilized silika ka vial khalase hlahisa e le tse tšoeu porous seponche. Kaha vial e feletseng tiisitsoe, lyophilized silika ka lula tsitsitseng bakeng sa nako e telele ea nako e (> likhoeli tse 18) ka mocheso kamoreng ka ho ya ka ponahalo, solubility, limolek'hule kabo boima le sebopeho. The nkeloa sebaka silika tharollo ntse e tšoanang mehaho phetoho bokgoni ho tloha kela tšohanyetso le α -helix sebopeho ho β -sheet sebopeho ka pele silika tharollo, e leng mahlonoko ba lokisetsa ho ea rulaganya tiileng tse bonahalang.
Tafole 1 paramethara, liteko le mokhoa oa lyophilized silika protheine
| Paramethara, Testing le Method | Lyophilized Silk protheine ena |
| Category | Thepa / Agents |
| foromo | mahlahana Lyophilized |
| Mohloli | Degummed Bombyx Mori tsoele |
| sephutheloana | 20 ML penicillin e Bottles |
| Mokhoa ea polokelo | Bolokwa ka o omileng, ventilated sebakeng ka wa kamore |
| Tharollo ea mahloriso ka mor'a rehydration | ≤ 40% |
| Bioburden | Sterilized |
| limolek'hule Weight | ≥ 200.000 Da |
| Letsatsi la pheletso | likhoeli tse 18 |
O mokhoa peration:
Lyophilized silika protheine ka ho bolokoa ka wa kamore bakeng sa likhoeli tse 18. Pele tšebeliso, metsi kapa Buffer kapa tharollo seleng ea / lithethefatsi ka eketsa ka nyopa khalase botlolo sebelisa nale ente (tsoela tiisitsoe) kapa pipette (bulehileng botlolo). The lyophilized silika kaka ka botlolo e ka potlako rehydrated eaba ba etsa khanya tharollo mosehla le concentrations ea silika a fumana ka fihla ho fihlela ho 30-40%.
A pplication :
Tšoanang e le tharollo ka mor'a tlhoekiso, e qhibilihang ka har'a lyophilized silika protheine ka boela sebelisoa e le lintho tse bonahalang ea motheo e tala bakeng sa popontshwa ea lihlahisoa tse ngata tsa biomedical. Ho feta moo, qhibilihang ka har'a lyophilized silika protheine e na le melemo ea nako e telele le a polokelo ambient, lipalangoang bonolo le mahloriso e phahameng ea tharollo nkeloa sebaka bapisa ho kamehla silika protheine tharollo.
